Western blot analysis of recombinant human IGF-1 (PHP177; reduced) probed with Rabbit anti Human insulin-like Growth factor I antibody (AHP1281) followed by the detection with HRP conjugated Goat anti Rabbit IgG (STAR208P) at 1/10,000. Different dilutions of AHP1281 are shown on lane 2 (0.2 ug/ml) and lane 4 (0.1 ug/ml). STAR208P only at a 1/10,000 dilution was included as a secondary only control (lane 6).